大鼠胸腺活化调节趋化因子(TARC)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗大鼠TARC抗体包被于酶标板上，实验时标本或标准品中的TARC会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠TARC抗体和辣根过氧化物酶标记的亲和素。抗大鼠TARC抗体与结合在包被抗体上的大鼠TARC结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），TARC浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中TARC的浓度。
大鼠胸腺活化调节趋化因子(TARC)化学发光**分析试剂盒描述：
| 英文名称 | Rat TARC (Thymus Activation Regulated Chemokine) CLIA Kit | ||
| 中文名称 | 大鼠胸腺活化调节趋化因子(TARC)化学发光**分析试剂盒 | ||
| 货号 | KL-R0643c | 种属 | Rat/大鼠 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 7.813~500pg/mL | 灵敏度 | 4.688pg/mL |
大鼠胸腺活化调节趋化因子(TARC)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 |
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| 产品说明书 | Manual | 1 份 |
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| 质检报告 | Certificate of Analysis | 1 份 |
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特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
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1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-IL1R1-Mu 小鼠白介素1受体Ⅰ(IL1R1) 检测试剂盒 mouse Interleukin 1 Receptor Type I (IL1R1) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 29pg/mL 96T 12 months
KL-IL1R2-Ra 大鼠白介素1受体Ⅱ(IL1R2) 检测试剂盒 Rat Interleukin 1 Receptor Type II (IL1R2) ELISA Kit Rattus norvegicus Rat 0.078-5 ng/mL 0.032 ng/mL 96T 12 months
KL-IL1R2-Mu 小鼠白介素1受体Ⅱ(IL1R2) 检测试剂盒 mouse Interleukin 1 Receptor Type II (IL1R2) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 6.4pg/mL 96T 12 months
KL-IL18-Hu 人白介素18(IL18) 检测试剂盒 human Interleukin 18 (IL18) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.3pg/mL 96T 12 months
KL-IL17-Ra 大鼠白介素17(IL17) 检测试剂盒 Rat Interleukin 17 (IL17) ELISA Kit Rattus norvegicus Rat 7.813-500pg/mL 3.1pg/mL 96T 12 months
KL-IL16-Ra 大鼠白介素16(IL16) 检测试剂盒 Rat Interleukin 16 (IL16) ELISA Kit Rattus norvegicus Rat 15.625-1000 pg/mL 6.85 pg/mL 96T 12 months
KL-IL15-Ra 大鼠白介素15(IL15) 检测试剂盒 Rat Interleukin 15 (IL15) ELISA Kit Rattus norvegicus Rat 15.625-1000pg/mL 5.4pg/mL 96T 12 months
KL-IL13-Ra 大鼠白介素13(IL13) 检测试剂盒 Rat Interleukin 13 (IL13) ELISA Kit Rattus norvegicus Rat 7.813-500pg/mL 3.3pg/mL 96T 12 months
KL-GHRH-Hu 人促生长**释放**(GHRH) 检测试剂盒 human Growth Hormone Releasing Hormone (GHRH) ELISA Kit Homo sapiens human 12.35-1000pg/mL 6.15pg/mL 96T 12 months
KL-GHRH-Mu 小鼠促生长**释放**(GHRH) 检测试剂盒 mouse Growth Hormone Releasing Hormone (GHRH) ELISA Kit Mus musculus mouse 12.35-1000pg/mL 4.34pg/mL 96T 12 months
KL-PTH-Ra 大鼠甲状旁腺**(PTH) 检测试剂盒 Rat Parathyroid Hormone (PTH) ELISA Kit Rattus norvegicus Rat 9.88-800pg/mL 4.25pg/mL 96T 12 months
KL-PTH-Mu 小鼠甲状旁腺**(PTH) 检测试剂盒 mouse Parathyroid Hormone (PTH) ELISA Kit Mus musculus mouse 9.88-800pg/mL 4.52pg/mL 96T 12 months
KL-NE-Ge 去甲肾上腺素(NE) 检测试剂盒 general Noradrenaline (NE) ELISA Kit General 61.73-5000pg/mL 27.2pg/mL 96T 12 months
KL-CTGF-Hu 人结缔组织生长因子(CTGF) 检测试剂盒 human Connective Tissue Growth Factor (CTGF) ELISA Kit Homo sapiens human 46.875-3000pg/mL 17.5pg/mL 96T 12 months
KL-CTGF-Ra 大鼠结缔组织生长因子(CTGF) 检测试剂盒 Rat Connective Tissue Growth Factor (CTGF) ELISA Kit Rattus norvegicus Rat 6.25-400pg/mL 2.46pg/mL 96T 12 months
KL-CTGF-Mu 小鼠结缔组织生长因子(CTGF) 检测试剂盒 mouse Connective Tissue Growth Factor (CTGF) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.061ng/mL 96T 12 months
