小鼠表皮脂肪酸结合蛋白5(FABP5)化学发光**分析试剂盒
基本原理:
本试剂盒采用双抗体夹心法。用抗小鼠FABP5抗体包被于酶标板上,实验时标本或标准品中的FABP5会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠FABP5抗体和辣根过氧化物酶标记的亲和素。抗小鼠FABP5抗体与结合在包被抗体上的小鼠FABP5结合、生物素与亲和素特异性结合而形成**复合物,游离的成分被洗去。加入发光底物混合液,发光底物在辣根过氧化物酶的催化下发出荧光,用化学发光**分析仪测定化学发光值(RLU),FABP5浓度与化学发光值之间呈正相关,通过绘制标准曲线求出标本中FABP5的浓度。
小鼠表皮脂肪酸结合蛋白5(FABP5)化学发光**分析试剂盒描述:
| 英文名称 | Mouse FABP5 (Fatty Acid Binding Protein 5, Epidermal) CLIA Kit | ||
| 中文名称 | 小鼠表皮脂肪酸结合蛋白5(FABP5)化学发光**分析试剂盒 | ||
| 货号 | KL-M0293c | 种属 | Mouse/小鼠 | 
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 31.25~2000pg/mL | 灵敏度 | 18.75pg/mL | 
小鼠表皮脂肪酸结合蛋白5(FABP5)化学发光**分析试剂盒试剂盒组成:
| 中文名称 | 英文名称 | 规格 | 保存 | 
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # | 
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # | 
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ | 
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # | 
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ | 
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) | 
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ | 
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ | 
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) | 
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) | 
| 封板覆膜 | Plate Sealer | 5 张 | 
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| 产品说明书 | Manual | 1 份 | 
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| 质检报告 | Certificate of Analysis | 1 份 | 
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					特别说明: #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃.  | 
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	1.在各孔中加入标准品或样品各 100μL,37℃孵育90分钟
2. 倒去孔内液体,拍干,加入 100μL  生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液, 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
	KL-IGF2-Gu	豚鼠胰岛素样生长因子2(IGF2) 检测试剂盒	 Guinea pig  Insulin Like Growth Factor 2 (IGF2) ELISA Kit	Cavia Guinea pig 	62.5-4000 pg/mL	21.4 pg/mL	96T	12 months
KL-IGFBP1-b	牛胰岛素样生长因子结合蛋白1(IGFBP1) 检测试剂盒	Bovine Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit	Bos taurus; Bovine  Cattle	0.781-50ng/mL	0.31ng/mL	96T	12 months
KL-IGFBP1-g	山羊胰岛素样生长因子结合蛋白1(IGFBP1) 检测试剂盒	 Goat Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit	Capra hircus; Caprine  Goat	3.125-200 ng/mL	1.45 ng/mL	96T	12 months
KL-IGFBP1-Fi	鱼胰岛素样生长因子结合蛋白1(IGFBP1) 检测试剂盒	fish Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit	Danio rerio  Zebrafish	0.156-10 ng/mL	0.086 ng/mL	96T	12 months
KL-Tie2-b	牛内皮细胞TEK酪氨酸激酶(Tie2) 检测试剂盒	Bovine TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit	Bos taurus; Bovine  Cattle	6.25-400 ng/mL	1.56 ng/mL	96T	12 months
KL-Tie2-Fi	鱼内皮细胞TEK酪氨酸激酶(Tie2) 检测试剂盒	fish TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit	Danio rerio  Zebrafish	1.563-100 ng/mL	0.34 ng/mL	96T	12 months
KL-Tie2-Mu	小鼠内皮细胞TEK酪氨酸激酶(Tie2)  检测试剂盒	mouse TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit	Mus musculus  mouse	0.156-10ng/mL	0.062ng/mL	96T	12 months
KL-TIMP2-b	牛组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	Bovine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Bos taurus; Bovine  Cattle	62.5-4000pg/mL	22.3pg/mL	96T	12 months
KL-TIMP2-c	犬组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Canis familiaris; Canine  Dog	6.25-400 ng/mL	1.5 ng/mL	96T	12 months
KL-TIMP2-Eq	马组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Equus caballus; Equine  Horse	3.125-200ng/mL	1.49ng/mL	96T	12 months
KL-TIMP2-Fi	鱼组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	fish Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Danio rerio  Zebrafish	1.563-100ng/mL	0.39ng/mL	96T	12 months
KL-TIMP2-Ch	鸡组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	Chicken Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Chicken  Gallus	7.813-500ng/mL	1.78ng/mL	96T	12 months
KL-TIMP2-Gu	豚鼠组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	 Guinea pig  Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Cavia Guinea pig 	1.563-100ng/mL	0.38ng/mL	96T	12 months
KL-TIMP2-p	猪组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒	porcine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit	Sus scrofa; Porcine  Pig	0.313-20ng/mL	0.103ng/mL	96T	12 months
    


