重组小鼠β-防御素3蛋白 - 上海康朗生物科技有限公司

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产品名称：重组小鼠β-防御素3蛋白
产品型号：rMuBD-3
产品厂商：KALANG
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简单介绍：

重组小鼠β-防御素3蛋白与其它公司提供的重组蛋白不同，rMuBD-3蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠β-防御素3蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

详情介绍：

重组小鼠β-防御素3蛋白

Synonyms Defensin beta3

Species Murine

Accession Q9WTL0

GeneID 27358

Source Escherichia coli.

Molecular Weight 重组小鼠β-防御素3蛋白Approximately 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.

Quantity 5µg/20µg/1000µg

AA Sequence KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重组小鼠β-防御素3蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by anti-microbial activity against E.coli. is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin 重组小鼠β-防御素3蛋白Less than 1 EU/μg of rMuBD-3 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重组小鼠β-防御素3蛋白

SDS-PAGE

Reference 1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.
2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.
3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,

Background Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

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Synonyms	Defensin beta3
Species	Murine
Accession	Q9WTL0
GeneID	27358
Source	Escherichia coli.
Molecular Weight	重组小鼠β-防御素3蛋白Approximately 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.
Quantity	5µg/20µg/1000µg
AA Sequence	KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组小鼠β-防御素3蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by anti-microbial activity against E.coli. is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin	重组小鼠β-防御素3蛋白Less than 1 EU/μg of rMuBD-3 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组小鼠β-防御素3蛋白
SDS-PAGE
Reference	1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91. 2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41. 3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,
Background	Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

产品名称：重组小鼠β-防御素3蛋白