产品详情
                        
                        
            简单介绍:
        
        
            重组鼠骨髓素/ CX3CL1蛋白与其它公司提供的重组蛋白不同,rMuFractalkine/CX3CL1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠骨髓素/ CX3CL1蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
        
    
            详情介绍:
        
        重组鼠骨髓素/ CX3CL1蛋白
| Species | Murine | 
| Accession | O35188 | 
| GeneID | 20312 | 
| Source | Escherichia coli. | 
| Molecular Weight | 重组鼠骨髓素/ CX3CL1蛋白Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Murine Fractalkine. | 
| Quantity | 5µg/20µg/1000µg | 
| AA Sequence | QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG | 
| Purity | > 97 % by SDS-PAGE and HPLC analyses. | 
| Biological Activity | 重组鼠骨髓素/ CX3CL1蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg. | 
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | 
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. | 
| Endotoxin | Less than 0.1 EU/µg of rMuFractalkine/CX3CL1 as determined by LAL method. | 
| Reconstitution | 重组鼠骨髓素/ CX3CL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. | 
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. | 
| 重组鼠骨髓素/ CX3CL1蛋白 | 
 
    


