神经营养因子4(NT4)重组蛋白
神经营养因子4(NT4)重组蛋白
Organism species
Mus musculus (Mouse)
Product No.
klA107Mu01
Source
Prokaryotic expression
Host
E.coli
Purity
> 95%
UOM
50ug
Predicted Molecular Mass
17.7kDa
Concentration
9.3
Applications
SDS-PAGE; WB; ELISA; IP.
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Residues
Gly80~Ala209 (Accession # Q80VU4) with two N-terminal Tags, His-tag and T7-tag
Formulation
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G VSETAPASRR GELAVCDAVS GWVTDRRTAV DLRGREVEVL GEVPAAGGSP LRQYFFETRC KAESAGEGGP GVGGGGCRGV DRRHWLSECK AKQSYVRALT ADSQGRVGWR WIRIDTACVC TLLSRTGRA
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
