人L1细胞粘附分子(L1CAM/CD171)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗人L1CAM/CD171抗体包被于酶标板上，实验时标本或标准品中的L1CAM/CD171会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人L1CAM/CD171抗体和辣根过氧化物酶标记的亲和素。抗人L1CAM/CD171抗体与结合在包被抗体上的人L1CAM/CD171结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），L1CAM/CD171浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中L1CAM/CD171的浓度。
人L1细胞粘附分子(L1CAM/CD171)化学发光**分析试剂盒描述：
| 英文名称 | Human L1CAM/CD171 (L1-Cell Adhesion Molecule) CLIA Kit | ||
| 中文名称 | 人L1细胞粘附分子(L1CAM/CD171)化学发光**分析试剂盒 | ||
| 货号 | KL-H1170c | 种属 | Human/人 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 31.25~2000pg/mL | 灵敏度 | 18.75pg/mL |
人L1细胞粘附分子(L1CAM/CD171)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 |
|
| 产品说明书 | Manual | 1 份 |
|
| 质检报告 | Certificate of Analysis | 1 份 |
|
|
特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
|||
1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-ANP-Hu 人心钠肽(ANP) 检测试剂盒 human Atrial Natriuretic Peptide (ANP) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.7pg/mL 96T 12 months
KL-AIF-Ra 大鼠凋亡诱导因子(AIF) 检测试剂盒 Rat Apoptosis Inducing Factor (AIF) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.061ng/mL 96T 12 months
KL-MUC5B-Hu 人粘蛋白5B(MUC5B) 检测试剂盒 human Mucin 5 Subtype B (MUC5B) ELISA Kit Homo sapiens human 0.625-40ng/mL 0.237ng/mL 96T 12 months
KL-PRL-Rb 兔催乳素(PRL) 检测试剂盒 Rabbit Prolactin (PRL) ELISA Kit Oryctolagus cuniculus Rabbit 0.313-20ng/mL 0.109ng/mL 96T 12 months
KL-CYPB-Hu 人亲环素B(CYPB) 检测试剂盒 human Cyclophilin B (CYPB) ELISA Kit Homo sapiens human 78.125-5000pg/mL 32pg/mL 96T 12 months
KL-INHBP-Ra 大鼠抑制素结合蛋白(INHBP) 检测试剂盒 Rat Inhibin Binding Protein (INHBP) ELISA Kit Rattus norvegicus Rat 0.781-50 ng/mL 0.192 ng/mL 96T 12 months
KL-IFNb-Ch 鸡干扰素β(IFNβ) 检测试剂盒 Chicken Interferon Beta (IFNb) ELISA Kit Chicken Gallus 15.625-1000pg/mL 5.7pg/mL 96T 12 months
KL-AGTR1-Hu 人血管紧张素Ⅱ受体1(AGTR1) 检测试剂盒 human Angiotensin II Receptor 1 (AGTR1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.055ng/mL 96T 12 months
KL-IL6-c 犬白介素6(IL6) 检测试剂盒 Canine Interleukin 6 (IL6) ELISA Kit Canis familiaris; Canine Dog 15.625-1000pg/mL 5.5pg/mL 96T 12 months
KL-IL10-c 犬白介素10(IL10) 检测试剂盒 Canine Interleukin 10 (IL10) ELISA Kit Canis familiaris; Canine Dog 15.625-1000pg/mL 5.5pg/mL 96T 12 months
KL-IFNg-c 犬干扰素γ(IFNγ) 检测试剂盒 Canine Interferon Gamma (IFNg) ELISA Kit Canis familiaris; Canine Dog 15.625-1000pg/mL 5.9pg/mL 96T 12 months
KL-PIIICP-Hu 人Ⅲ型前胶原羧基端原肽(PⅢCP) 检测试剂盒 human Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit Homo sapiens human 12.5-800pg/mL 5.3pg/mL 96T 12 months
KL-PIICP-Hu 人Ⅱ型前胶原羧基端原肽(PⅡCP) 检测试剂盒 human Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit Homo sapiens human 31.25-2000pg/mL 13.6pg/mL 96T 12 months
KL-IGF2R-Hu 人胰岛素样生长因子2受体(IGF2R) 检测试剂盒 human Insulin Like Growth Factor 2 Receptor (IGF2R) ELISA Kit Homo sapiens human 0.625-40ng/mL 0.244ng/mL 96T 12 months
KL-LTBP2-Hu 人组织内转化生长因子β结合蛋白2(LTBP2) 检测试剂盒 human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.062ng/mL 96T 12 months
KL-ACTa1-Hu 人骨骼肌肌动蛋白α1(ACTα1) 检测试剂盒 human Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.118ng/mL 96T 12 months
KL-GLUT3-Hu 人葡萄糖转运蛋白3(GLUT3) 检测试剂盒 human Glucose Transporter 3 (GLUT3) ELISA Kit Homo sapiens human 0.781-50ng/mL 0.32ng/mL 96T 12 months
KL-TSC22-Hu 人转化生长因子β刺激蛋白22(TSC22) 检测试剂盒 human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.113ng/mL 96T 12 months
