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QA-bio QA-bio

QA-bioFor O-linked glycan release, the Ludger Liberate Orela Kit allows the release of glycans that have free reducing terminii suitable for fluorescent labelling.QA-bio The kit is straightforward and contains a simple method with no special handling techniques as required by the alternative method, hydrazinolysis.

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QAbio  Product categories


Endoglycosidases

Exoglycosidases

Deglycosylation Kits

Sialic Acid Products

Glycan Labeling

Capture and Cleanup

Glycan Standards

Columns and Buffers

New Products



QAbio  Glycoprofiling

Glycoprofiling is used by the biopharmaceutical industry to analyze the glycans found on glycoproteins being developed as therapeutics. It is also a key assay for the lot release characterization of manufactured glycoproteins both before and after approval.

The process can be broken down as follows:

  • Release of glycans from glycoprotein
  • capture and cleanup of released glycans
  • labeling of released glycans
  • purification and cleanup of labeled glycans
  • analysis of glycan using MS, HPLC, or CE to produce the glycan profile

QAbio  Glycan Release

Typically, PNGase F is used to release N-linked glycans. The enzyme works quickly and effectively, cleaving the glycans in native conditions in most cases. The Endo F family of enzymes are also commonly used for glycan release. These three enzymes vary in specificity to the multi-antennary structures found on N-linked glycans. They also differ from PNGase F in that they leave the penultimate glycan, a charged N-acetyl-glucosamine, attached to the protein. Both PNGase F and the Endo F family of enzymes leave the glycan with a free reducing terminii suitable for fluorescent labelling.

For O-linked glycan release, the Ludger Liberate Orela Kit allows the release of glycans that have free reducing terminii suitable for fluorescent labelling. The kit is straightforward and contains a simple method with no special handling techniques as required by the alternative method, hydrazinolysis. 

  • PNGase F

    $433.00
  • Endo F Multi-Kit

    $433.00
  • Orela O-linked Glycan Release Kit

    $973.00

QAbio  Glycan Capture and Cleanup

A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents.

  • LudgerClean EB10 cartridges have been designed for purification of glycans from proteins, salts, and detergents.
  • The LudgerClean PBM Post-Glycosidase Clean-Up plate has been developed for removal of glycosidases prior to MS analysis in a 96-well format.
  • LudgerClean CEX cartridges purify O-glycans from non-carbohydrate material including salts, proteins, and detergents.
  • CEX Cartridges for O-glycans

    $283.00
  • EB10 Cartridges

    $314.00
  • PBM Glycosidase Removal

    $271.00

QAbio  Glycan Labeling

Glycans are then typically labeled with a reporter molecule based upon the separation/detection systems being used for the analysis. Ludger has developed a variety of robust and reliable glycan labeling kits.

Fluorescent molecules 2-AB and 2-AA have been used for glycan analysis in HPLC assays for many years and are considered a gold standard within the industry.

Permethylation labeling kits are available for mass spectrometry and APTS have been developed for capillary electrophoresis.

Procainamide labelling permits glycan identification by either mass spectrometry or UHPLC, and because of its improved ionisation efficiency it can permit identification of minor glycans (>1% relative peak area) by ESI-MS.

  • Procainamide Labeling Kit

    $757.00
  • 2-AB Labeling Kit with Non-Toxic Reductant

    $539.00
  • 2-AB Labeling Kit

    $539.00
  • 2-AA Labeling Kit

    $539.00

QAbio  HPLC Columns and Buffers

We supply a full line of Ludger HPLC columns and buffers developed specifically for glycan analysis.

  • C2 and C3 SAX columns and buffer – charge based analysis
  • N1 and N2 Normal phase columns and buffer – size based analysis
  • R1, R2, and uR2 columns and solvent – sialic acid and monosacchride analysis
  • N2 4.6mm Column

    $3,326.00
  • Placeholder

    LS-N Buffer

    $230.00
  • C2 x50mm SAX Column

    $2,244.00

QAbio  Glycan and Glycoprotein Standards

QA-Bio also supplies a wide variety of qualitative and quantitative glycan standards and glycoprotein standards to be used during glycoprofiling. All are produced at the highest quality and purity levels.

  • Glycans – higherer than 90% pure by NMR and HPLC
  • Glycan Libraries – Fetuin, IgG
  • Quantitative standards – determined by qNMR and qMonosaccharide analysis
  • Glycoprotein Standards – Fetuin, IgG
  • Procainamide

    Procainamide

  • APTS Labeled

    APTS Labeled

  • 2-AA Labeled

    2-AA Labeled

  • 2-AB Labeled

    2-AB Labeled

  • O-Linked

    O-Linked

  • N-Linked

    N-Linked

  • Glycoproteins

    Glycoproteins

  • Glycan Libraries

    Glycan Libraries

  • Glycans

    Glycans

  • Quantitative

    Quantitative




DMB Sialic Acid Labeling Kit

The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acIDS.

The kit includes all the reagents to release sialic acids from your glycoprotein and label these with DMB.

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

$670.00

uR2 2.1x100mm UHPLC Column

Developed for the analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.

Dimensions: 2.1mm x 100mm

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x100

$1,577.00

Sialic Acid Quantitation Kit

The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).

View product documentation as PDF

Part Number: KE-SIALIQ

$512.00

Sialic Acid Aldolase

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation as PDF

Part Number: E-ALD01

$433.00

CMP-Sialic Acid Synthetase

This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.

View product documentation as PDF

Part Number: E-CMP01

$433.00




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